1. Examination of cell signaling pathway induced by different type of physical effort.
The main goal of the project is to assess the impact of 2 types of physical effort, namely aerobic and anaerobic, on the expression level of genes encoding hypoxia inducible factors HIF-1-alpha and HIF-2-alpha in peripheral blood nucleated cells and the level of these proteins in the vascular bed. Studies also include mechanisms for regulating the level of gene expression. It is planned to determine the role of proteasome in regulating the level of analyzed transcription factors at the protein level, as well as their role as modulators inducing repair processes and/or activating autophagy and apoptosis pathways in peripheral blood nuclear cells.
2. Application of in silico research for new models in basic research (synthetic biology).
The main goal of this research is to assess the pseudogenization of selected genes (e.g. humanin) in a wide taxonomic panel, optimize synonymic codons in the context of understanding the metabolic role of selected proteins, and search for tools with completely new research functionalities, allowing to design new experimental models suitable in medical biology.